Chevron Left
Zurück zu Basics of Extracellular Vesicles

Kursteilnehmer-Bewertung und -Feedback für Basics of Extracellular Vesicles von University of California, Irvine

4.8
Sterne
241 Bewertungen
61 Bewertungen

Über den Kurs

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

Top-Bewertungen

MH

Jan 11, 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

DD

May 17, 2020

I found Ia very professional way to introduce everyone to the amazing world of EV. Professor show elegantly their knowledge. The course fulfils my expectations. Congratulations! Nice works

Filtern nach:

26 - 50 von 59 Bewertungen für Basics of Extracellular Vesicles

von Sherri C

Dec 12, 2016

Excellent course. A great overview plus a lot of detailed information that can't be found by reading the literature.

von Preeti S

Jun 15, 2020

It is amazing course ,I got to know much about extracellular vesicle and the quiz have also very good questions.

von Laura E S S

Aug 05, 2019

Considero que los temas están muy bien resumidos y son fáciles de entender, los tiempos son flexibles.

von Thijs G

Sep 29, 2016

I found this a very informative course on the recent developments and the basics of the EV field.

von Shiyou C

Feb 13, 2017

The course provide a general introduction of this field and the methods usde in the experiments.

von Elizabeth R F

Jul 01, 2019

This course was so amazing! It was so hard but I learned so much! Good job Cecilia Laaser!

von Lorena L R V

May 29, 2017

Excelente curso, reafirma los conceptos básicos, da consejos para las distintas técnicas.

von Francielton d S L

Sep 08, 2019

It was a great experience, i was able to know more about it and apply it to my research.

von Geraldo M J

Jun 23, 2020

Um curso incrível!!!! Aprendi o necessário para o meu trabalho de iniciação científica.

von Thitaporn S

Dec 26, 2019

This course provides enough essential information for study of extracellular vesicles.

von Jess M

Sep 14, 2016

Aprendí mucho. Muy completo, aunque pudieron haber profundizado un poco más.

von LIN, T

Mar 02, 2020

Great course to learn the basic knowledges of extracellular vesicles!

von Asmaa A M A

May 14, 2020

Excellent course, totally enjoyed it and benefited alot from it

von Laura M M E

May 04, 2020

Excelente curso, expositores con amplio conocimiento del tema

von Stephanie B

Oct 05, 2019

Good basic overview for someone starting in their studies

von Wenhao L

Apr 26, 2017

Very wonderful courses! Better than reading 100 papers!

von Mohammad R

Feb 05, 2017

This course gives an excellent overview of the field!

von Nahla I S H

Apr 22, 2020

A very good course, really it was very informative.

von pbmansfield

Mar 06, 2017

Well put together and very informative. Great job!

von Dr.� S R S A P -

Jun 26, 2020

very helpful and nice videos and the contents too

von Olivia C

Aug 06, 2020

very useful course, I really enjoy it!

von Yingchao M

Apr 09, 2020

Very good for beginners.

von Abner M N

Nov 22, 2019

Amazing course!

von Adel D

Nov 03, 2016

very good!

von Kaio M R

Oct 10, 2017

ÓTIMO