If you express that gene in the hex cell, [FOREIGN] channel properties. [FOREIGN] This work is really important, okay? [FOREIGN] So if you found this [INAUDIBLE] is important channel but here's they are only in their [INAUDIBLE] does not relates to physiology, right? So you still need actually examine whether this channel is responsible for your detection of the touch. So what can you do, then you need to do to knock out, right? Indeed, actually this group the Piezo2 Is responsible for the light touch. Just a gentle touch this is actually, we talk about actually, okay? A light touch could be found a megacell that could be found, right? Just like you can, [FOREIGN] you get axon as you connect with that. [FOREIGN] Now what they did, [FOREIGN]. [FOREIGN] This neuron gives a action potential but when the [FOREIGN] condition [FOREIGN]. A mucous cell [FOREIGN] okay? They found actually you can tell this wild type and this condition in north up. The responses are different, right? This kind of continuous response here but there's still some transient response here. So it tells you that actually, light touch can detect by both the neutral cell and the neuron. [FOREIGN] This is the experiments of information so for this make a note are channel are mainly in the mammalian system. Right now, this family Piezo Is the most studied channels, okay? So for the somatosensation, as we mentioned, we still have the temperature sensation, right? And for the temperature, and actually, there is another family called the TRP channel. The TRP Channel. The TRP channel is quite a unique family. You see they are six transmembering domain protein and the NC terminal. Both inside the cellular site, this is a channel, okay? So the discovery of this channel, also as Tanya mentioned. [FOREIGN] Those root ganglion cell, [FOREIGN] mechanical capsizing [FOREIGN]. [FOREIGN] then they use the cast imaging to detect whether you apply the sizing. You will get some response, okay? If you found, and then you can narrow down that data pool. That pool and then you say, [FOREIGN] you will have a more efficient transaction, right? [FOREIGN] channel [FOREIGN] capsizing, okay? [FOREIGN] If they express the [FOREIGN] channel in the [FOREIGN]. What they found is actually you use capsizing stimulation, you'll get huge results. At the same time, if you increase the temperature by 43 degrees, then you also get a huge response. Now, from here, the data tells you this TRP E1 channel has dual functions. It can sense the chemical of capsaicin, it also can sense the temperature stimulation. And through a similar strategy they found actually another channel called trip M8. Trip M8 is a different channel, okay? They responded to the constant stimulation [FOREIGN] and also responded to the main cell. The main cell, [FOREIGN] okay? So, the triple m 8 channel responded to code and the main sub, okay? If you express this two together then you will respond to auto stimulation, so the temperature sensation. Mainly through the TRP channel family, okay? So again, if these two channels doesn't mean they really detect the temperature of your skin, right? And then the knockout, okay? They knock out for example when the [FOREIGN] channel here is this is the wild type respondent to the 45 degree. [FOREIGN] now and then if you have TRPM8 knockout the animal's still able to respond to the 45 degree stimulation. That means TRPM8 not responsible for the sensation but TRPU1 knockout they lost a huge response, okay? Majority was the response a lot due to the triple one knock out,also for the count stimulation now [FOREIGN]. Considers coat stimulation for the sale and the while type is okay, so we are supposed to 22 degrees stimulation. But not to the triple M8 knock out, so the triple M8 is responsible for the [INAUDIBLE] .okay? Of course this is in the mouse but it's quite interesting, okay. There are so other channels are not discovered yet because, in this radio they have, like only 12 degrees stimulation. [FOREIGN] You see, all this actually essentially similar, the same. [FOREIGN] That means there must be some other channels responsible for detection of these that got really low temperature. And also, again actually, for the high temperatures, we still have some here left, that means maybe some other channels. So people are still current activity to look for this temperature sensing channel, okay?
